Primer Design and In Silico Amplification of PhtD Gene from Streptococcus pneumoniae as Independent Serotype Pneumoniae Protein Vaccine Candidate

Article Sidebar

PDF
Published: Apr 9, 2023
DOI: https://doi.org/10.56855/jhsp.v1i2.283
Keywords:
In silico, pneumonia, primary, streptococcus pneumonia, vaccine

Main Article Content

Risma Wiharyanti
Universitas Negeri Yogyakarta
Fera Aulia
Universitas Negeri Yogyakarta
Astiningdiah Merdekawati
PT. Bio Farma

Abstract

Purpose: The aim of this study was to obtain a primer design capable of amplifying the PhtD gene from various S. pneumoniae bacterial serotypes as a protein-based pneumonia vaccine candidate that does not depend on bacterial serotypes.
Methods: Primary preparation was carried out through a bioinformatics approach using several software followed by testing the use of primers in the amplification process which was carried out in silico.
Results: The results of this study were a pair of primers capable of amplifying the PhtD gene from several S. pneumoniae serotypes.
Conclusion: The PhtD gene of the 6 serotypes of S. pneumoniae has high similarities, making it suitable for use as a protein-based vaccine candidate. Based on the consensus of the six serotypes, a pair of primers was obtained that were able to amplify 17 S. pneumoniae strains from several serotypes contained in the reference sequence in silico.

Article Details

How to Cite
Wiharyanti, R., Aulia, F., & Merdekawati, A. (2023). Primer Design and In Silico Amplification of PhtD Gene from Streptococcus pneumoniae as Independent Serotype Pneumoniae Protein Vaccine Candidate. Consilium Sanitatis: Journal of Health Science and Policy, 1(2), 98–106. https://doi.org/10.56855/jhsp.v1i2.283
Issue
Vol. 1 No. 2 (2023): April
Section
Articles

References

Parameswarappa SG, Reppe K, Geissner A, Ménová P, Govindan S, Calow ADJ, et al. A Semi-synthetic Oligosaccharide Conjugate Vaccine Candidate Confers Protection against Streptococcus pneumoniae Serotype 3 Infection. Cell Chem Biol. 2016;23(11):1407–16.

André GO, Assoni L, Rodriguez D, Leite LCC, dos Santos TEP, Ferraz LFC, et al. Immunization with PhtD truncated fragments reduces nasopharyngeal colonization by Streptococcus pneumoniae. Vaccine. 2020;38(26):4146–53.

Daniels CC, Rogers PD, Shelton CM. A review of pneumococcal vaccines: Current polysaccharide vaccine recommendations and future protein antigens. J Pediatr Pharmacol Ther. 2016;21(1):27–35.

Feldman C. Review: Current and new generation pneumococcal vaccines. Vol. 69, Journal of Infection. 2014. p. 309–25.

Santiesteban-Lores LE, Cabrera-Crespo J, Carvalho E. Development of a pneumococcal conjugate vaccine based on chemical conjugation of polysaccharide serotype 6B to PspA. Microb Pathog. 2021;158(April):105092.

Geno KA. Pneumococcal capsules and their types: Past, present, and future. Clin Microbiol Rev. 2015;28(3):871–99.

Masomian M, Ahmad Z, Gew LT, Poh CL. Development of next generation streptococcus pneumoniae vaccines conferring broad protection. Vaccines. 2020;8(1):1–23.

Oliveira GS, Oliveira MLS, Miyaji EN, Rodrigues TC. Pneumococcal vaccines: Past findings, present work, and future strategies. Vaccines. 2021;9(11).

Ayu Safitri T, Nurul Jannah Patty D, Saraswati H. 16 dan 18 Sebagai Dasar dalam Desain Primer untuk Deteksi Kanker Leher Rahim dengan In-house. IJOBB. 2018;2(2):67.

Saraswati H. Analisa Bioinformatika Gen E1 dan E2 Dari Virus Hepatitis C (HCV) Genotipe 1, 2, 3 dan 6 Sebagai Kandidat Vaksin Viral- Like Particles (VLP). Indones J Biotechnol Biodivers. 2017;2(2):48–57.

Seprianto, Wahyuni FD. Analisis Bioinformatika Gen Potensial Penyandi Halichondrin B Dari Spons Laut Sebagai Kandidat Anti. Indones J Biotechnol Biodivers. 2018;2(2):57–66.

Mardalisa, Suhandono S, Yanti N, Rozi F, Nova F, Primawati. Bioinformatic analysis in designing mega-primer in overlap extension PCR cloning (OEPC) technique. Int J Informatics Vis. 2021;5(2):139–43.